Microwave-Assisted Synthesis of Nesfatin and AFP-6: An Efficient Tool for Synthesis of Long and Challenging Peptide Sequences.
Ved P. Srivastava and Fernando J. Ferrer
Amylin Pharmaceuticals Inc., San Diego, CA, USA

Microwave-assisted synthesis has become increasingly advantageous in solid phase peptide synthesis (SPPS). This technology resolves problems such as intermolecular aggregation, ß-sheet formation and steric hindrance from amino acid protecting groups during peptide synthesis. Synthesis of longer and more challenging peptides such as human Nesfatin (82 amino acid residues) and Amylin Family Peptide (47 amino acid residues), AFP-6, using microwave technique will be discussed.