Ether Precipitation
Ice cold ether added to the
peptide dissolved in TFA will lead to precipitation
of the peptide from the TFA solution. Typically,
diethyl or t-butyl methyl ether are used. Peroxide-free
ether should be used for precipitation. For
each 1mL of TFA solution, add 10mL of ether
for complete precipitation.
 |
 |
| Diethyl
Ether |
t-butyl
methyl ether (MTBE) |
|
