| COMBINE CHEMMATRIX RESINS WITH MICROWAVE ENERGY FOR HIGH
PURITY PEPTIDE SYNTHESIS! |
www.matrix-innovation.com |
www.cempeptides.com |
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CHEMMATRIX RESINS
- Improved swelling characteristics over polystyrene
- Allow for faster reagent penetration
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PEG Based |
PS
Based |
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MICROWAVE SYNTHESIS- Polar
or ionic species will attempt to align with the alternating electric
component of a microwave
- Aggregation can be overcome since the peptide backbone
is polar
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Microwave Energy disrupting aggregation |
d-LACTAM FORMATION DURING ARGININE COUPLING PREVENTS COUPLING |
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SYNTHESIS EXAMPLE- ABRF 1992 TEST PEPTIDE: |
| Gly-Val-Arg-Gly-Asp-Lys-Gly-Asn-Pro-Gly-Trp-Pro-Gly-Ala-Pro-Tyr |
Major Arginine deletion |
Major Arginine deletion |
Synthesis Conditions- Non-microwave
- Tyr(tBu)-Wang Resin (0.88meq/g)
- Deprotection = 20% Piperidine in DMF
- Coupling = HBTU/DIEA, 5-fold excess
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Synthesis Conditions
- Microwave
- Tyr(tBu)-Wang Resin (0.88meq/g)
- Deprotection = 20% Piperidine in DMF
- Coupling = HBTU/DIEA, 5-fold excess
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- No Arginine Deletion
- Total Synthesis=7.25h
- Complete deprotection w/ Piperazine
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Synthesis Conditions
- Microwave (7.25 hours)
- PAL-ChemMatrix Resin (0.6meq/g)
- Deprotection = 5% Piperazine w/ 0.1M HOBt in DMF
- Coupling = HBTU/DIEA, 5-fold excess
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| Best Result – using ChemMatrix Resin with Microwave |
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