Best in class technology
- Single-Mode microwave cavity with fiber-optic temperature measurement offers the latest and best in microwave technology
- Hydrolyze hydrophobic proteins more efficiently without excessive
degradation to sensitive residues: serine and threonine.
- Vapor phase hydrolysis allows only the acid vapor and its
condensate to hydrolyze the protein, thus eliminating contamination due
to the hydrolyzing acid.
- Samples remain in the same HPLC vial for hydrolysis and analysis – improving
accuracy and sensitivity.
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Components of the 10-place 300mL vessel holder for use in the
Discover microwave |
Ease of use - Load up to ten 300µL sample tubes at one time
- The same chemistry can be used with microwave as conventional
methods
- Microwave hydrolysis allows for fast and efficient reaction
times
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Somatostatin
(MW 1678 D)
AGCLNFFWKTFTSC |
| |
Lit |
24 hrs
110°C |
90 min
150°C |
Microwave
20-25 mins
150°C |
| Asp |
1 |
1.1 |
1.1 |
1.1 |
| Ser |
1 |
1.0 |
1.0 |
0.9 |
| Gly |
1 |
1.0 |
1.0 |
1.0 |
| Thr |
2 |
2.1 |
1.9 |
1.8 |
| Cys |
2 |
1.9 |
2.0 |
1.9 |
| Ala |
1 |
1.0 |
1.0 |
1.0 |
| Phe |
3 |
3.1 |
3.0 |
2.8 |
| Lys |
2 |
1.9 |
1.9 |
1.8 |
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- Vessel holder can be easily prepared for the hydrolysis step in the microwave system.
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| Protein Hydrolysis vessel assembly |
Flexibility - Optional software available for recording temperature/pressure graphs
- System can easily fit in a fume hood
- System can be used to accelerate enzymatic digestion of
proteins and peptide from hours to minutes
- System can be configured to synthesize difficult peptides
with cycle times of 25-minute
|
(a) |
(b)
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Optional accessories available for (a)- enzymatic digestion using up to fourteen 1.5mL eppendorf
tubes and (b)- solid phase peptide synthesis |
Service - 1-year full warranty standard with the system
- Simple system with no moving parts
- Various level service contracts available to fit your
needs
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